Beilstein J. Org. Chem.2020,16, 3038–3051, doi:10.3762/bjoc.16.253
compared to Skyline, and GlycopeptideGraphMS. All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control. Partial cysteineoxidation was identified as an unexpectedly abundant peptide modification and impaired the
quantification.
Keywords: bioinformatics; cysteineoxidation; glycoproteomics; immunoglobulins; mass spectrometry; Introduction
Protein glycosylation mainly occurs in the form of N- and O-glycosylation. N-Glycans are attached to Asn within an amino acid consensus sequence (Asn-Xxx-Ser/Thr, Xxx ≠ Pro) and O
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Graphical Abstract
Figure 1:
Integration of automated glycopeptide identification by Byonic and GlycopeptideGraphMS (aided by Op...